Journal: Molecular Medicine Reports
Article Title: Anti-angiogenic therapy for normalization of tumor vasculature: A potential effect of Buyang Huanwu decoction on nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential
doi: 10.3892/mmr.2016.4854
Figure Lengend Snippet: Immunohistochemical staining of the expression of HIF-1α in hepatocellular carcinoma tissues from nude mice of different groups on different days (3,3-diaminobenzidine staining; original magnification, ×400). LM, model control; LB, Buyang Huanwu decotion-treated; LY, Yiqi decoction-treated; LH, Huoxue decoction-treated.
Article Snippet: The slides were then incubated with either primary VEGF monoclonal mouse antibody (clone VG1; M7273; DAKO; 1:100 dilution) for 60 min at 37°C, primary rabbit ant-mouse RGS-5 polyclonal antibody (SAB1411523; Sigma-Aldrich; 1:80 dilution) overnight at 4°C, or primary rabbit anti-mouse HIF-1α polyclonal antibody (sc-10790; Santa Cruz Biotechnology, Inc., Dallas, TX, USA; 1:200 dilution) overnight at 4°C.
Techniques: Immunohistochemical staining, Staining, Expressing, Control